Astroglial Boundary Formation and EphA4 Signaling in Neuroblast Migration
نویسنده
چکیده
................................................................................................................................... 6 Introduction............................................................................................................................ 7 Materials and Methods......................................................................................................... 19 Animals.................................................................................................................................. 19 Immunohistochemistry...................................................................................................... 19 RMS Area Measurements.................................................................................................... 20 BrdU Immunohistochemistry.............................................................................................. 20 BrdU/EdU Pulse Labeling.................................................................................................... 21 MACS/FACS Cell Enrichment and Sorting........................................................................... 22 qRT-‐PCR................................................................................................................................. 23 Results.................................................................................................................................... 24 Astrocyte Proliferation and Infiltration into the Neuroblast-‐Dense RMS..................... 24 Astrocyte Morphology in the Rostral Migratory Stream Resembles Reactive Astrogliosis..................................................................................................................................................... 27 EphA4 Plays a Crucial Role in Glial Tube Formation and Neuroblast Restriction in the RMS.................................................................................................................................................................... 29 Identification of EphA4-‐Expressing Cells Using a Novel EphA4-‐ΔeGFP MACS-‐FACS Approach......................................................................................................................................................... 33 The Role of EphA4 Signaling in Regulating Proliferation in the SVZ/RMS System.................................................................................................................................. 36 Conclusions............................................................................................................................ 39 References.............................................................................................................................. 43
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